BioBits blog

Byte-sized insights for modern bioinformatics

Introducing the qPCR Primer Analyzer ILVO

Introducing the qPCR Primer Analyzer ILVO, a web-based tool for evaluating primer/probe sets for qPCR assays with salt-corrected Tm calculations and secondary structure detection.

2 min read · August 20, 2025

2025 · qPCR in-silico html bioinformatics genomics · bioinformatics genomics tools
Batch In-Silico (q)PCR Made Easy with run_epcr.py

Python script for batch in-silico PCR and probe finding across folders of genome FASTA files. Supports IUPAC ambiguity codes, fuzzy matching, multi-threading, and outputs TSV tables, FASTA amplicons, and amplicon count matrices.

3 min read · August 06, 2025

2025 · PCR in-silico python bioinformatics genomics · bioinformatics genomics tools
From Bash to Copilot: My Bioinformatics Terminal Workflow

How I use WSL, tmux, SSH, VSCode, and GitHub Copilot to streamline bioinformatics research and development.

11 min read · July 27, 2025

2025 · WSL tmux vscode github-copilot productivity linux · bioinformatics terminal