🧬 Precision Meets Simplicity

In the ever-evolving field of bioinformatics, precision and usability are paramount. That’s why I developed the qPCR Primer Analyzer ILVO, a streamlined yet powerful web-based tool designed to support researchers in evaluating oligonucleotide sequences for qPCR assays.

🧪 What It Does

The qPCR Primer Analyzer ILVO helps users assess the quality and compatibility of primer/probe sets with a focus on:

• Salt-Corrected Melting Temperature (Tm): Ensures accurate thermodynamic calculations by factoring in sodium and magnesium concentrations.
• Hairpin and Cross-Dimer Detection: Identifies potential secondary structures that could compromise assay performance.
• Thermo Fisher Guideline Compatibility: Aligns with industry standards for robust and reproducible results.

⚙️ How It Works

Users can input:

• Forward and reverse primers (5’ → 3’)
• Optional probe sequences
• Ion concentrations (Na⁺ and Mg²⁺)
• Primer concentration (nM)

With a single click on 🔬 Analyze Sequences, the tool delivers a comprehensive analysis of the primer set’s thermodynamic properties and structural integrity.

🧑‍🔬 Built for Researchers, by a Researcher

Created at ILVO – Flanders Research Institute for Agriculture, Fisheries and Food, this tool is open-source and licensed under the https://opensource.org/licenses/MIT. It’s designed to be intuitive for molecular biologists while offering the depth needed by bioinformaticians.

📫 Get in Touch

Have feedback or want to collaborate? Reach out at [steve.baeyen@ilvo.vlaanderen.be]

💻 Clone or download the html file from my repository on ILVO Gitlab